This course teaches fundamental methodologies in omics-based systems biology including (1) Type, structure, and characteristics of biological systems (single molecules, molecular complexes, organelles, cells, tissues, organs, circulation systems, etc.), (2) omics (genomics, transcriptomics, epigenomics, proteomics, metabolomics, etc.) technologies, (3) standard data formats for exchange and storage and data normalization and visualization, (4) Identification of genes/proteins/metabolites predominantly expressed in a particular condition (e.g., diseases, stresses, and infections), clustering, and functional enrichment analysis, (5) Types of biological networks and network modeling and analysis, and (6) Identification of key regulators, pathways, or network modules governing a given phenotype. Case studies where these methodologies are applied to real biological questions will be introduced.